PSAT1 Monoklonaler Antikörper

PSAT1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B2C9

Kat-Nr. : 67619-1-PBS

Synonyme

1B2C9, EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA

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  WB analysis using 67619-1-Ig (same clone as 67619-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67619-1-Ig (PSAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67619-1-Ig (same clone as 67619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67619-1-Ig (PSAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 67619-1-Ig (same clone as 67619-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSAT1 antibody (67619-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67619-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PSAT1 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSAT1 fusion protein Ag14076
• Vollständiger Name: phosphoserine aminotransferase 1
• Berechnetes Molekulargewicht: 370 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC001618
• Gene symbol: PSAT1
• Gene ID (NCBI): 29968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740 )