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PSAT1 Rekombinanter Antikörper

PSAT1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1O22

Kat-Nr. : 81479-1-PBS

Synonyme

1O22, EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA

Filter:
  • Western Blot (WB) analysis of various lysates using PSAT1 Recombinant antibody (81479-1-RR)

    WB analysis using 81479-1-RR (same clone as 81479-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81479-1-RR (PSAT1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81479-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PSAT1 Recombinant antibody (81479-1-RR)

    WB analysis of HepG2 using 81479-1-RR (same clone as 81479-1-PBS)

    WB result of PSAT1 antibody (81479-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAT1 transfected HepG2 cells. This data was developed using the same antibody clone with 81479-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSAT1 Recombinant antibody (81479-1-RR)

    IF Staining of HepG2 using 81479-1-RR (same clone as 81479-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (81479-1-RR, Clone: 1O22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 81479-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

81479-1-PBS bindet in WB, IF/ICC, Indirect ELISA PSAT1 und zeigt Reaktivität mit Hausschwein, human, Maus

Getestete Reaktivität Hausschwein, human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PSAT1 fusion protein Ag0776
Vollständiger Name phosphoserine aminotransferase 1
Berechnetes Molekulargewicht 40 kDa
Beobachtetes Molekulargewicht 37-40 kDa
GenBank-ZugangsnummerBC004863
Gene symbol PSAT1
Gene ID (NCBI) 29968
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID: 18221502, 20955740)