PSD95-Specific Rekombinanter Antikörper

PSD95-Specific Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1I22

Kat-Nr. : 81106-1-PBS

Synonyme

PSD95-Specific,DLG4, DLG4, PSD-95, psd95/dlg4, 1I22

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  WB analysis using 81106-1-RR (same clone as 81106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81106-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PSD95-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: discs, large homolog 4 (Drosophila)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: NM_001365
• Gene symbol: PSD95
• Gene ID (NCBI): 1742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).