PSIP1 Monoklonaler Antikörper

PSIP1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9E7

Kat-Nr. : 67723-1-Ig

Synonyme

Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7

---

• 

  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 67723-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67723-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 67723-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67723-1-Ig bindet in WB, IF/ICC, ELISA PSIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSIP1 fusion protein Ag30562
• Vollständiger Name: PC4 and SFRS1 interacting protein 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_021144
• Gene symbol: PSIP1
• Gene ID (NCBI): 11168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.