PSIP1 Monoklonaler Antikörper

PSIP1 Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9E7

Kat-Nr. : 67723-1-PBS

Synonyme

1E9E7, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF

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  WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67723-1-Ig (same clone as 67723-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP50375-1

  The mean PSIP1 concentration was determined to be 89.0 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load, 25.7 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 99.2 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load and 26.7 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP50375-1

  Sandwich ELISA standard curve of MP50375-1, Human PSIP1 Monoclonal Matched Antibody Pair - PBS only. 67723-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag21886. 68941-1-PBS was HRP conjugated as the detection antibody. Range: 93.4-6000 pg/mL.

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  IF Staining of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50375-1

  Cytometric bead array standard curve of MP50375-1, PSIP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67723-1-PBS. Detection antibody: 68941-1-PBS. Standard:Ag21886. Range: 3.125-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

67723-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA PSIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSIP1 fusion protein Ag30562
• Vollständiger Name: PC4 and SFRS1 interacting protein 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_021144
• Gene symbol: PSIP1
• Gene ID (NCBI): 11168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.