PSIP1 Rekombinanter Antikörper

PSIP1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242841C5

Kat-Nr. : 85395-1-PBS

Synonyme

242841C5, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF

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  WB analysis using 85395-1-RR (same clone as 85395-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85395-1-RR (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85395-1-RR (same clone as 85395-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (85395-1-RR, Clone: 242841C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85395-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85395-1-RR against Human PSIP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85395-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PSIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PSIP1 fusion protein Ag21886
• Vollständiger Name: PC4 and SFRS1 interacting protein 1
• Berechnetes Molekulargewicht: 530 aa, 60 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC064135
• Gene symbol: PSIP1
• Gene ID (NCBI): 11168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.