PSMA2 Polyklonaler Antikörper

PSMA2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14377-1-AP

Synonyme

PSC3, Proteasome subunit alpha type-2, HC3

---

• 

  WB analysis of NIH/3T3 using 14377-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14377-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14377-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14377-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14377-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14377-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14377-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14377-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 14377-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14377-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 14377-1-AP

  Immunofluorescent analysis of HepG2 cells using 14377-1-AP (PSMA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen, HepG2-Zellen, Mausnierengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

14377-1-AP bindet in WB, IHC, IF/ICC, ELISA PSMA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMA2 fusion protein Ag5753
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC047697
• Gene symbol: PSMA2
• Gene ID (NCBI): 5683
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

Publikationen

Species	Application	Title
	WB	EMBO Rep; MAPL loss dysregulates bile and liver metabolism in mice; Authors - Vanessa Goyon
human	WB	Acta Biomater; Pushpin-like nanozyme for plasmon-enhanced tumor targeted therapy; Authors - Baofu Ma
human	IHC	J Ovarian Res; Development and clinical validation of a seven-gene signature based on tumor stem cell-related genes to predict ovarian cancer prognosis; Authors - Guangwei Wang
human	WB	J Gastroenterol; Corynoline protects chronic pancreatitis via binding to PSMA2 and alleviating pancreatic fibrosis; Authors - Pengyuan Wang