PSMA3 Polyklonaler Antikörper

PSMA3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11887-1-PBS

Synonyme

PSC8, Proteasome subunit alpha type-3, HC8

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  WB analysis of A549 using 11887-1-AP (same clone as 11887-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 11887-1-AP (same clone as 11887-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 11887-1-AP (same clone as 11887-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11887-1-AP (same clone as 11887-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11887-1-AP (same clone as 11887-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11887-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PSMA3 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMA3 fusion protein Ag2495
• Vollständiger Name: proteasome (prosome, macropain) subunit, alpha type, 3
• Berechnetes Molekulargewicht: 255 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC029402
• Gene symbol: PSMA3
• Gene ID (NCBI): 5684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.