PSMB11 Polyklonaler Antikörper

PSMB11 Polyklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55143-1-PBS

Synonyme

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  WB analysis of Jurkat using 55143-1-AP (same clone as 55143-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55143-1-AP (PSMB11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

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  IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

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  IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 55143-1-AP (same clone as 55143-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PSMB11 (55143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55143-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA PSMB11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: proteasome (prosome, macropain) subunit, beta type, 11
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: NM_001099780
• Gene symbol: PSMB11
• Gene ID (NCBI): 122706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.