PSMB4 Polyklonaler Antikörper

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• KD/KO Validated

PSMB4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11029-1-AP

Synonyme

20S proteasome, 26 kDa prosomal protein, HN3, HsBPROS26, HsN3, Macropain beta chain, PROS 26, PROS26, Proteasome beta chain, Proteasome chain 3, Proteasome subunit beta type 4, PSMB4

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Galerie der Validierungsdaten

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  WB analysis using 11029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11029-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11029-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11029-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11029-1-AP (PSMB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11029-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11029-1-AP (PSMB4 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 11029-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PSMB4 antibody 11029-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 11029-1-AP

  Immunofluorescent analysis of HepG2 cells using 11029-1-AP (PSMB4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Plazenta-Gewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11029-1-AP bindet in WB, IHC, IF, ELISA PSMB4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMB4 fusion protein Ag1500
• Vollständiger Name: proteasome (prosome, macropain) subunit, beta type, 4
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC010088
• Gene symbol: PSMB4
• Gene ID (NCBI): 5692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB4(Proteasome subunit beta type-4) is also named as PROS26 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The human protein PSMB4 is known mainly as an interaction partner for several proteins, suggesting an important function of binding not only to the lid, but also to the 20 S core of the proteasome. PSMB4 might be a major site for proteasome regulation, where signals from the outside might be transduced to the protease activities inside(PMID:15660529 ). The full length 30 kDa protein has a propeptide of 45 amino acid.

Publikationen

Species	Application	Title
human	WB	Cancer Res; Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes.; Authors - Genee Y Lee
mouse	WB	J Biol Chem; Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes; Authors - Xinzhi Li
human	WB,IHC	Oncol Rep; PSMB4 overexpression enhances the cell growth and viability of breast cancer cells leading to a poor prognosis.; Authors - Hua Wang ; KD Validated
human,pig,monkey	WB,IF	J Virol; PSMB4 Degrades the Porcine Reproductive and Respiratory Syndrome Virus Nsp1α Protein via the Autolysosome Pathway and Induces the Production of Type I Interferon; Authors - Heyou Yi ; KD Validated
mouse	WB	JHEP Rep; Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice; Authors - Chunhong Liu