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PSMB6 Rekombinanter Antikörper

PSMB6 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251232G5

Kat-Nr. : 86491-1-PBS

Synonyme

EC:3.4.25.1, LMPY, Proteasome subunit beta type-6, Y

Filter:
  • Western Blot (WB) analysis of various lysates using PSMB6 Recombinant antibody (86491-1-RR)

    WB analysis using 86491-1-RR (same clone as 86491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86491-1-RR (PSMB6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMB6 Recombinant antibody (86491-1-RR)

    IF Staining of HeLa using 86491-1-RR (same clone as 86491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB6 antibody (86491-1-RR, Clone: 251232G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86491-1-RR

    Affinity and Kinetic Characterization of 86491-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86491-1-RR against Human PSMB6 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

86491-1-PBS bindet in WB, IF/ICC, Indirect ELISA PSMB6 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PSMB6 fusion protein Ag2296
Vollständiger Name proteasome (prosome, macropain) subunit, beta type, 6
Berechnetes Molekulargewicht 239 aa, 25 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC000835
Gene symbol PSMB6
Gene ID (NCBI) 5694
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).