PSMB7 Polyklonaler Antikörper

PSMB7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14727-1-AP

Synonyme

20S proteasome, Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7

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  WB analysis of Raji using 14727-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14727-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14727-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

14727-1-AP bindet in WB, IHC, ELISA PSMB7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMB7 fusion protein Ag6486
• Vollständiger Name: proteasome (prosome, macropain) subunit, beta type, 7
• Berechnetes Molekulargewicht: 29.9 kDa
• Beobachtetes Molekulargewicht: 25-27 kDa
• GenBank-Zugangsnummer: BC000509
• Gene symbol: PSMB7
• Gene ID (NCBI): 5695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction.; Authors - Samir R Nath
rat	WB	Int J Mol Sci; Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.; Authors - Wenjuan Zhang
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie