PSMB9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMB9 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 14544-1-AP

Synonyme

20S proteasome, beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12

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Galerie der Validierungsdaten

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  WB analysis using 14544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14544-1-AP (PSMB9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14544-1-AP (PSMB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 14544-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14544-1-AP (LMP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14544-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14544-1-AP (LMP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14544-1-AP

  IP Result of anti-LMP2 (IP:14544-1-AP, 4ug; Detection:14544-1-AP 1:300) with Raji cells lysate 2800ug.

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  IHC staining of human spleen using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14544-1-AP (PSMB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of HepG2 using 14544-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14544-1-AP (LMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Mauslebergewebe, Mauslungengewebe, Mausmilzgewebe, Mausnierengewebe, Raji-Zellen, Rattenlebergewebe, Rattenmilzgewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

14544-1-AP bindet in WB, IP, IHC, IF, ELISA PSMB9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMB9 fusion protein Ag6048
• Vollständiger Name: proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC065513
• Gene symbol: PSMB9
• Gene ID (NCBI): 5698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.

Publikationen

Species	Application	Title
human	WB	Mol Cell; DNA damage promotes HLA class I presentation by stimulating a pioneer round of translation-associated antigen production.; Authors - Yuki Uchihara ; KD Validated
human	IF	Cell Res; In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures.; Authors - Chao Ni
mouse		EMBO J; Proteasomal degradation within endocytic organelles mediates antigen cross-presentation.; Authors - Debrup Sengupta
human	WB	Cancer Lett; Deciphering the tumor-suppressive role of PSMB9 in melanoma through multi-omics and single-cell transcriptome analyses; Authors - Xing Hu
mouse	WB	Cell Rep; The portrait of liver cancer is shaped by mitochondrial genetics.; Authors - Mrittika Chattopadhyay
human	IF,WB	Front Oncol; Immune Characteristics and Prognosis Analysis of the Proteasome 20S Subunit Beta 9 in Lower-Grade Gliomas.; Authors - Junzhe Liu