PSMC1 Polyklonaler Antikörper

PSMC1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11196-1-AP

Synonyme

P26S4, p56, PSMC1, S4

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 11196-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11196-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11196-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11196-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

11196-1-AP bindet in WB, ELISA PSMC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMC1 fusion protein Ag1677
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, ATPase, 1
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 52-57 kDa
• GenBank-Zugangsnummer: BC016368
• Gene symbol: PSMC1
• Gene ID (NCBI): 5700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMC1(26S protease regulatory subunit 4) is also named as p26s4 and belongs to the AAA ATPase family.The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Publikationen

Species	Application	Title
human,mouse	WB	Sci Adv; Lipid-anchored proteasomes control membrane protein homeostasis; Authors - Ruizhu Zhang
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
		Cell Mol Life Sci; The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S.; Authors - Diego Sbardella
human	WB	PLoS One; Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.; Authors - Jayashree Chadchankar
rat	WB	Neuropathology; Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo.; Authors - Watabe Kazuhiko K