PSMC1 Polyklonaler Antikörper

PSMC1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 11196-1-AP

Synonyme

P26S4, 26S proteasome regulatory subunit 4, 26S proteasome AAA-ATPase subunit RPT2

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  WB analysis of mouse brain using 11196-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11196-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11196-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11196-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 11196-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11196-1-AP (PSMC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 11196-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 11196-1-AP (PSMC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

11196-1-AP bindet in WB, IHC, ELISA PSMC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMC1 fusion protein Ag1677
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, ATPase, 1
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 52-57 kDa
• GenBank-Zugangsnummer: BC016368
• Gene symbol: PSMC1
• Gene ID (NCBI): 5700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMC1(26S protease regulatory subunit 4) is also named as p26s4 and belongs to the AAA ATPase family.The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Publikationen

Species	Application	Title
human,mouse	WB	Sci Adv; Lipid-anchored proteasomes control membrane protein homeostasis; Authors - Ruizhu Zhang
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
		Cell Mol Life Sci; The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S.; Authors - Diego Sbardella
human	WB	PLoS One; Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.; Authors - Jayashree Chadchankar
rat	WB	Neuropathology; Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo.; Authors - Watabe Kazuhiko K
human	WB	Cell Death Dis; Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib; Authors - Yu Feng