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PSMC1 Rekombinanter Antikörper

PSMC1 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251014B9

Kat-Nr. : 86237-1-PBS

Synonyme

26S proteasome AAA-ATPase subunit RPT2, 26S proteasome regulatory subunit 4, P26S4

Filter:
  • Western Blot (WB) analysis of various lysates using PSMC1 Recombinant antibody (86237-1-RR)

    WB analysis using 86237-1-RR (same clone as 86237-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86237-1-RR (PSMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PSMC1 Recombinant antibody (86237-1-RR)

    IHC staining of mouse cerebellum using 86237-1-RR (same clone as 86237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86237-1-RR (PSMC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PSMC1 Recombinant antibody (86237-1-RR)

    IF Staining of Jurkat using 86237-1-RR (same clone as 86237-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMC1 antibody (86237-1-RR, Clone: 251014B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86237-1-RR

    Affinity and Kinetic Characterization of 86237-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86237-1-RR against Human PSMC1 were performed. The affinity constant is 0.402 nM.

Geprüfte Anwendungen

Produktinformation

86237-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PSMC1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PSMC1 fusion protein Ag1677
Vollständiger Name proteasome (prosome, macropain) 26S subunit, ATPase, 1
Berechnetes Molekulargewicht 49 kDa
Beobachtetes Molekulargewicht52-60 kDa
GenBank-ZugangsnummerBC016368
Gene symbol PSMC1
Gene ID (NCBI) 5700
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.