HOP2 Polyklonaler Antikörper

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• KD/KO Validated

HOP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11339-1-AP

Synonyme

GT198, HOP2, HUMGT198A, PSMC3 interacting protein, PSMC3IP, TBP 1 interacting protein, TBPIP

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Galerie der Validierungsdaten

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  WB analysis using 11339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11339-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11339-1-AP

  IP Result of anti-HOP2 (IP:11339-1-AP, 4ug; Detection:11339-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of mouse testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11339-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSMC3IP antibody 11339-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

11339-1-AP bindet in WB, IP, IHC, IF, ELISA HOP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOP2 fusion protein Ag1883
• Vollständiger Name: PSMC3 interacting protein
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 24 kDa and 29 kDa
• GenBank-Zugangsnummer: BC008792
• Gene symbol: PSMC3IP
• Gene ID (NCBI): 29893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMC3IP, also called HOP2, encodes a protein that functions in meiotic recombination. It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. The protein encoded by this gene can also co-activate ligand-driven transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in this gene cause XX female gonadal dysgenesis.

Publikationen

Species	Application	Title
human	WB	Cell; Interchromosomal Homology Searches Drive Directional ALT Telomere Movement and Synapsis.; Authors - Nam Woo Cho ; KD Validated
mouse	IF	Nucleic Acids Res; RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis; Authors - Chao Liu
human	WB	Cell Rep; MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells; Authors - Anabel Zelceski
human		Nat Commun; Promotion of homology-directed DNA repair by polyamines.; Authors - Chih-Ying Lee
human	WB,IF	Oncotarget; A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).; Authors - Matthew Tsang