PSMC5 Polyklonaler Antikörper

PSMC5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14752-1-AP

Synonyme

p45, p45/SUG, Proteasome subunit p45, PSMC5, Rpt6, S8, SUG1, TBP10, TRIP1

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  WB analysis using 14752-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14752-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14752-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14752-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14752-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14752-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, Jurkat-Zellen, Mausnierengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14752-1-AP bindet in WB, IHC, ELISA PSMC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMC5 fusion protein Ag6497
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, ATPase, 5
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 45-49 kDa
• GenBank-Zugangsnummer: BC002367
• Gene symbol: PSMC5
• Gene ID (NCBI): 5705
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.