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PSMD11 Rekombinanter Antikörper

PSMD11 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251499B5

Kat-Nr. : 86596-3-PBS

Synonyme

26S proteasome non-ATPase regulatory subunit 11, p44.5, Rpn6, S9

Filter:
  • Western Blot (WB) analysis of various lysates using PSMD11 Recombinant monoclonal antibody (86596-3-RR)

    WB analysis using 86596-3-RR (same clone as 86596-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86596-3-RR (PSMD11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PSMD11 Recombinant monoclonal antibody (86596-3-RR)

    IHC staining of mouse testis using 86596-3-RR (same clone as 86596-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86596-3-RR (PSMD11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMD11 Recombinant monoclonal antibody (86596-3-RR)

    IF Staining of HeLa using 86596-3-RR (same clone as 86596-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD11 antibody (86596-3-RR, Clone: 251499B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86596-3-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PSMD11 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PSMD11 fusion protein Ag6435
Vollständiger Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
Berechnetes Molekulargewicht 47 kDa
Beobachtetes Molekulargewicht 47 kDa
GenBank-ZugangsnummerBC000437
Gene symbol PSMD11
Gene ID (NCBI) 5717
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.