PSMD14/POH1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMD14/POH1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12059-1-AP

Synonyme

PAD1, POH1, PSMD14, PSMD14/POH1, rpn11

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Galerie der Validierungsdaten

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  WB analysis of A431 using 12059-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 12059-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12059-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12059-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A375 using 12059-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (12059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A375 using 12059-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using PSMD14/POH1 antibody (12059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, K-562-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

12059-1-AP bindet in WB, IHC, IF, ELISA PSMD14/POH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD14/POH1 fusion protein Ag2694
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC009524
• Gene symbol: PSMD14
• Gene ID (NCBI): 10213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.

Publikationen

Species	Application	Title
human	WB,IF	Sci Transl Med; Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.; Authors - Angel S Byrd
human	WB, IHC, IF	Nat Commun; POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.; Authors - Boshi Wang ; KD Validated
human	WB	Theranostics; Blockade of deubiquitinating enzyme PSMD14 overcomes chemoresistance in head and neck squamous cell carcinoma by antagonizing E2F1/Akt/SOX2-mediated stemness.; Authors - Chao Jing ; KD Validated
human	WB, IF	Theranostics; The PSMD14 inhibitor Thiolutin as a novel therapeutic approach for esophageal squamous cell carcinoma through facilitating SNAIL degradation.; Authors - Chao Jing
human	WB	Clin Transl Med; MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.; Authors - Mengqing Xiao ; KD Validated
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A