PSMD14/POH1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSMD14/POH1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 12059-1-AP

Synonyme

PSMD14, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome-associated PAD1 homolog 1, EC:3.4.19.-, PAD1

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  WB analysis using 12059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 12059-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12059-1-AP

  WB result of PSMD14/POH1 antibody (12059-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD14/POH1 transfected A431 cells.

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  WB analysis using 12059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12059-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12059-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A375 using 12059-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (12059-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, MDA-MB-468-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

12059-1-AP bindet in WB, IHC, IF/ICC, ELISA PSMD14/POH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMD14/POH1 fusion protein Ag2694
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC009524
• Gene symbol: PSMD14
• Gene ID (NCBI): 10213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11; Authors - Bing Zhou
human	WB,IF	Sci Transl Med; Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.; Authors - Angel S Byrd
human	WB,IHC,IF	Nat Commun; POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.; Authors - Boshi Wang ; KD Validated
human	WB,IHC	Cancer Lett; POH1 induces Smad3 deubiquitination and promotes lung cancer metastasis; Authors - Yang Yuan ; KD Validated
human	WB	Theranostics; Blockade of deubiquitinating enzyme PSMD14 overcomes chemoresistance in head and neck squamous cell carcinoma by antagonizing E2F1/Akt/SOX2-mediated stemness.; Authors - Chao Jing ; KD Validated
human	WB,IF	Theranostics; The PSMD14 inhibitor Thiolutin as a novel therapeutic approach for esophageal squamous cell carcinoma through facilitating SNAIL degradation.; Authors - Chao Jing