PSMD14/POH1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PSMD14/POH1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242160E7

Kat-Nr. : 84767-3-RR

Synonyme

PSMD14, 242160E7, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome-associated PAD1 homolog 1, EC:3.4.19.-

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  WB analysis using 84767-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 84767-3-RR

  WB result of PSMD14/POH1 antibody (84767-3-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD14/POH1 transfected A431 cells.

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  IHC staining of mouse heart using 84767-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 84767-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 84767-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (84767-3-RR, Clone: 242160E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84767-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84767-3-RR against Human PSMD14/POH1 were performed. The affinity constant is 3.60 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, Mausherzgewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84767-3-RR bindet in WB, IHC, IF/ICC, ELISA PSMD14/POH1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PSMD14/POH1 fusion protein Ag2694
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC009524
• Gene symbol: PSMD14
• Gene ID (NCBI): 10213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.