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PSMD3 Rekombinanter Antikörper

PSMD3 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242470D4

Kat-Nr. : 84928-2-PBS

Synonyme

242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3

Filter:
  • Western Blot (WB) analysis of various lysates using PSMD3 Recombinant antibody (84928-2-RR)

    WB analysis using 84928-2-RR (same clone as 84928-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PSMD3 Recombinant antibody (84928-2-RR)

    WB analysis using 84928-2-RR (same clone as 84928-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMD3 Recombinant antibody (84928-2-RR)

    IHC staining of human colon cancer using 84928-2-RR (same clone as 84928-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84928-2-RR (PSMD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMD3 Recombinant antibody (84928-2-RR)

    IHC staining of human colon cancer using 84928-2-RR (same clone as 84928-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84928-2-RR (PSMD3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMD3 Recombinant antibody (84928-2-RR)

    IF Staining of HeLa using 84928-2-RR (same clone as 84928-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PSMD3 Recombinant antibody (84928-2-RR)

    IF Staining of U-251 using 84928-2-RR (same clone as 84928-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMD3 Recombinant antibody (84928-2-RR)

    IF Staining of U2OS using 84928-2-RR (same clone as 84928-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PSMD3 Recombinant antibody (84928-2-RR)

    IF Staining of A549 using 84928-2-RR (same clone as 84928-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using PSMD3 Recombinant antibody (84928-2-RR)

    FC experiment of A549 using 84928-2-RR (same clone as 84928-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PSMD3 Recombinant antibody (84928-2-RR)

    FC experiment of HeLa using 84928-2-RR (same clone as 84928-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01691-2

    Cytometric bead array standard curve of MP01691-2

    Cytometric bead array standard curve of MP01691-2, PSMD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84928-2-PBS. Detection antibody: 84928-1-PBS. Standard: Ag2684. Range: 0.781-100 ng/mL.

Geprüfte Anwendungen

Produktinformation

84928-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA PSMD3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PSMD3 fusion protein Ag2684
Vollständiger Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Berechnetes Molekulargewicht 534 aa, 61 kDa
Beobachtetes Molekulargewicht 61 kDa
GenBank-ZugangsnummerBC012302
Gene symbol PSMD3
Gene ID (NCBI) 5709
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).