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PSMD4 Rekombinanter Antikörper

PSMD4 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251460G8

Kat-Nr. : 86558-1-PBS

Synonyme

26S proteasome non-ATPase regulatory subunit 4, AF, ASF, MCB1, Multiubiquitin chain-binding protein

Filter:
  • Western Blot (WB) analysis of various lysates using PSMD4 Recombinant monoclonal antibody (86558-1-RR)

    WB analysis using 86558-1-RR (same clone as 86558-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86558-1-RR (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PSMD4 Recombinant monoclonal antibody (86558-1-RR)

    IHC staining of mouse testis using 86558-1-RR (same clone as 86558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86558-1-RR (PSMD4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86558-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMD4 Recombinant monoclonal antibody (86558-1-RR)

    IF Staining of HeLa using 86558-1-RR (same clone as 86558-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD4 antibody (86558-1-RR, Clone: 251460G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86558-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86558-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PSMD4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PSMD4 fusion protein Ag6691
Vollständiger Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Berechnetes Molekulargewicht 41 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC002365
Gene symbol PSMD4
Gene ID (NCBI) 5710
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell-permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895)