PSMD5 Monoklonaler Antikörper

PSMD5 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5F7

Kat-Nr. : 67682-1-PBS

Synonyme

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

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  WB analysis of pig liver using 67682-1-Ig (same clone as 67682-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67682-1-Ig (same clone as 67682-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 67682-1-Ig (same clone as 67682-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit liver using 67682-1-Ig (same clone as 67682-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67682-1-Ig (same clone as 67682-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67682-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PSMD5 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSMD5 fusion protein Ag19298
• Vollständiger Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
• Berechnetes Molekulargewicht: 504 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC014478
• Gene symbol: PSMD5
• Gene ID (NCBI): 5711
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.