PSME1 Polyklonaler Antikörper

PSME1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10543-1-PBS

Synonyme

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  WB analysis of mouse spleen using 10543-1-AP (same clone as 10543-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10543-1-AP (same clone as 10543-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 10543-1-AP (same clone as 10543-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10543-1-AP (same clone as 10543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10543-1-AP (same clone as 10543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10543-1-PBS bindet in WB, IHC, Indirect ELISA PSME1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSME1 fusion protein Ag0831
• Vollständiger Name: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29-33 kDa
• GenBank-Zugangsnummer: BC007503
• Gene symbol: PSME1
• Gene ID (NCBI): 5720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

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