PSME2 Polyklonaler Antikörper

PSME2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12937-2-PBS

Synonyme

REG-beta, REGbeta, REG beta, PA28beta, PA28B

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  WB analysis of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12937-2-AP (same clone as 12937-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 12937-2-AP (same clone as 12937-2-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

  IP result of anti-PSME2 (IP:12937-2-AP, 4ug; Detection:12937-2-AP 1:500) with MCF-7 cells lysate 2000ug. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME2 antibody (12937-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12937-2-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PSME2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSME2 fusion protein Ag3986
• Vollständiger Name: proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
• Berechnetes Molekulargewicht: 239 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC019885
• Gene symbol: PSME2
• Gene ID (NCBI): 5721
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.