PSME3 Monoklonaler Antikörper

PSME3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A8E8

Kat-Nr. : 67629-1-PBS

Synonyme

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

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  WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67629-1-PBS bindet in WB, IHC, ELISA PSME3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PSME3 fusion protein Ag6716
• Vollständiger Name: proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC001423
• Gene symbol: PSME3
• Gene ID (NCBI): 10197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.