PSME4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PSME4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18799-1-PBS

Synonyme

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  WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

  IP result of anti-PSME4 (IP:18799-1-AP, 3ug; Detection:18799-1-AP 1:500) with HEK-293 cells lysate 1760ug. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18799-1-AP (same clone as 18799-1-PBS)

  Immunofluorescent analysis of Hela cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18799-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PSME4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: proteasome (prosome, macropain) activator subunit 4
• Berechnetes Molekulargewicht: 211 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC113668
• Gene symbol: PSME4
• Gene ID (NCBI): 23198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.