PTBP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PTBP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

39

Kat-Nr. : 12582-1-AP

Synonyme

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

---

Galerie der Validierungsdaten

• 

  WB analysis using 12582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12582-1-AP

  WB result of PTBP1 antibody (12582-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

• 

  WB analysis of rat heart using 12582-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12582-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12582-1-AP (PTBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 12582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, BxPC-3-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Mauslungengewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Pankreasgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 32 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• RIP: See 4 publications below

Produktinformation

12582-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA PTBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTBP1 fusion protein Ag3277
• Vollständiger Name: polypyrimidine tract binding protein 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC013694
• Gene symbol: PTBP1
• Gene ID (NCBI): 5725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10

Publikationen

Species	Application	Title
human	WB	Nat Cancer; SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers; Authors - Lei Shi
human	WB	Nat Cell Biol; Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2; Authors - Haissi Cui
human	RIP	Blood; circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.; Authors - Yu-Meng Sun
human	WB	Nat Commun; Systematic characterization of cancer transcriptome at transcript resolution; Authors - Wei Hu
rat	WB	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma