PTBP1 Monoklonaler Antikörper

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• KD/KO Validated

PTBP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D6D8

Kat-Nr. : 67462-1-PBS

Synonyme

3D6D8, HNRNP I, HNRNPI, HNRPI, pPTB

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  WB analysis using 67462-1-Ig (same clone as 67462-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67462-1-Ig (PTBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67462-1-Ig (same clone as 67462-1-PBS)

  WB result of PTBP1 antibody (67462-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 67462-1-Ig (PTBP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67462-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PTBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTBP1 fusion protein Ag28404
• Vollständiger Name: polypyrimidine tract binding protein 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC013694
• Gene symbol: PTBP1
• Gene ID (NCBI): 5725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.