PTCD3 Polyklonaler Antikörper

PTCD3 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 25158-1-AP

Synonyme

PTCD3, TRG 15

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  WB analysis of mouse embryo using 25158-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 25158-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 25158-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25158-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 25158-1-AP

  IP result of anti-PTCD3 (IP:25158-1-AP, 4ug; Detection:25158-1-AP 1:600) with HEK-293 cells lysate 3600ug.

• 

  IF Staining of HEK-293 using 25158-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25158-1-AP (PTCD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausembryogewebe, HEK-293-Zellen, Rattenherzgewebe, Rattennierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

25158-1-AP bindet in WB, IF/ICC, IP, CoIP, RIP, ELISA PTCD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTCD3 fusion protein Ag18330
• Vollständiger Name: Pentatricopeptide repeat domain 3
• Berechnetes Molekulargewicht: 689 aa, 79 kDa
• Beobachtetes Molekulargewicht: 78-80 kDa
• GenBank-Zugangsnummer: BC011832
• Gene symbol: PTCD3
• Gene ID (NCBI): 55037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
human	WB	Cell Death Dis; MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness.; Authors - Seongki Min
human	WB	Biomedicines; Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema; Authors - Loukmane Karim
human	WB	J Gen Virol; Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors; Authors - Shabab Chowdhury
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia
human	RIP,CoIP	FASEB J; Epigenetic Activation of PTCD3 Promotes CRC Glutamine Metabolism and Metastasis via IGF2BP2-Mediated SLC38A2 m6A Modification; Authors - Weihui Peng