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PTDSS1 Polyklonaler Antikörper

PTDSS1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20820-1-PBS

Synonyme

PSS1, EC:2.7.8.29, KIAA0024, Phosphatidylserine synthase 1, PSS-1

Filter:
  • Western Blot (WB) analysis of various lysates using PTDSS1 Polyclonal antibody (20820-1-AP)

    WB analysis using 20820-1-AP (same clone as 20820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20820-1-AP (PTDSS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PTDSS1 Polyclonal antibody (20820-1-AP)

    IHC staining of human placenta using 20820-1-AP (same clone as 20820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20820-1-AP (PTDSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTDSS1 Polyclonal antibody (20820-1-AP)

    IF Staining of HeLa using 20820-1-AP (same clone as 20820-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTDSS1 antibody (20820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTDSS1 Polyclonal antibody (20820-1-AP)

    IF Staining of HepG2 using 20820-1-AP (same clone as 20820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTDSS1 antibody (20820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20820-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20820-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PTDSS1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PTDSS1 fusion protein Ag14170
Vollständiger Name phosphatidylserine synthase 1
Berechnetes Molekulargewicht 473 aa, 56 kDa
Beobachtetes Molekulargewicht38~40 kDa
GenBank-ZugangsnummerBC002376
Gene symbol PTDSS1
Gene ID (NCBI) 9791
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTDSS1 gene encodes the enzyme phosphatidylserine synthase 1 (PSS1), which catalyzes the production of phosphatidylserine (PS), a membrane phospholipid involved in different cellular processes such as development, cell signaling, apoptosis, and blood coagulation (PMID: 35224839). PTDSS1 is highly enriched in mitochondria-associated membranes (MAM) and is largely excluded from the bulk of the endoplasmic reticulum (ER). Low apparent molecular mass of 40 kDa compared with the size predicted was attributed to the exceptionally high content of hydrophobic amino acids in PSS1 (PMID: 10938271).