PTDSS1 Rekombinanter Antikörper

PTDSS1 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240007G3

Kat-Nr. : 83421-1-RR

Synonyme

PSS1, PSS-1, Phosphatidylserine synthase 1, KIAA0024, EC:2.7.8.29

---

• 

  IF Staining of Hela using 83421-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Hela cells using PTDSS1 antibody (83421-1-RR, Clone: 240007G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 83421-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PTDSS1 Recombinant antibody (83421-1-RR, Clone:240007G3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83421-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83421-1-RR against Human PTDSS1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83421-1-RR bindet in IF/ICC, FC (Intra), ELISA PTDSS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PTDSS1 fusion protein Ag14170
• Vollständiger Name: phosphatidylserine synthase 1
• Berechnetes Molekulargewicht: 473 aa, 56 kDa
• GenBank-Zugangsnummer: BC002376
• Gene symbol: PTDSS1
• Gene ID (NCBI): 9791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphatidylserine Synthase-1 (PTDSS1 or PSS1) is one of two enzymes involved in the production of phosphatidylserine (PS), which is a quantitatively minor, but physiologically important phospholipid in mammalian cells (PMID: 24241535). Mice lacking either Ptdss1 or Ptdss2 are viable, phenotypically normal and fertile, indicating that, in mice, PSS1 and PSS2 can compensate for each other (PMID: 18343815).