PTGDS Polyklonaler Antikörper

PTGDS Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10754-2-AP

Synonyme

Beta trace protein, Cerebrin 28, LPGDS, PDS, PGD2, PGD2 synthase, PGDS, PGDS2, Prostaglandin D2 synthase, Prostaglandin H2 D isomerase, PTGDS

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10754-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10754-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10754-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10754-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10754-2-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10754-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, humanes Kolongewebe, humanes Nierengewebe, humanes Uringewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

10754-2-AP bindet in WB, IHC, ELISA PTGDS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTGDS fusion protein Ag1163
• Vollständiger Name: prostaglandin D2 synthase 21kDa (brain)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC005939
• Gene symbol: PTGDS
• Gene ID (NCBI): 5730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGDS(Prostaglandin-H2 D-isomerase) is also named as PTGDS, PDS, PGDS2. It catalyzes the conversion of PGH2 to PGD2, and is a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. It has two N-glycosylation recognition sites.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway.; Authors - Hui-Yuan Su
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
human	WB	Cell Cycle; MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling.; Authors - Ailan Yin
human	IHC	Cancer Manag Res; Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation.; Authors - Ruoyao Zou
human	WB	Med Sci Monit; Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.; Authors - Kim Jae Ho JH
mouse	IHC	Mol Neurodegener; MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease; Authors - Lu Geng