PTGDS Polyklonaler Antikörper

PTGDS Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10754-2-PBS

Synonyme

LPGDS, Lipocalin-type prostaglandin-D synthase, Glutathione-independent PGD synthase, EC:5.3.99.2, Cerebrin-28

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  WB analysis of HeLa using 10754-2-AP (same clone as 10754-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  WB analysis using 10754-2-AP (same clone as 10754-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 10754-2-AP (same clone as 10754-2-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 10754-2-AP (same clone as 10754-2-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10754-2-AP (same clone as 10754-2-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 10754-2-AP (same clone as 10754-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10754-2-AP (same clone as 10754-2-PBS)

  IP result of anti-PTGDS (IP:10754-2-AP, 4ug; Detection:10754-2-AP 1:600) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10754-2-AP (same clone as 10754-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10754-2-AP (same clone as 10754-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10754-2-AP (same clone as 10754-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10754-2-AP (same clone as 10754-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10754-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10754-2-PBS bindet in WB, IHC, IP, Indirect ELISA PTGDS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTGDS fusion protein Ag1163
• Vollständiger Name: prostaglandin D2 synthase 21kDa (brain)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC005939
• Gene symbol: PTGDS
• Gene ID (NCBI): 5730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycoprotein prostaglandin D2 synthase (PTGDS), also known as L-PGDS, PGDS, and LPGDS, is a member of the lipocalin superfamily and plays dual roles in prostaglandins metabolism and lipid transport (PMID: 34743203). PTGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PTGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. PTGDS is abundantly expressed in the brain and heart and has also been found in testis, prostate, kidney, spleen and leukocytes. It can be found as a collection of monomeric glycoforms with molecular weights in the 27-34 kDa range (PMID: 32430846).