PTGER4 Monoklonaler Antikörper

PTGER4 Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A2A12

Kat-Nr. : 66921-1-PBS

Synonyme

4A2A12, EP4, EP4R

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  WB analysis of pig brain using 66921-1-Ig (same clone as 66921-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66921-1-Ig (same clone as 66921-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66921-1-Ig (same clone as 66921-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66921-1-Ig (same clone as 66921-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66921-1-Ig (same clone as 66921-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PTGER4 (66921-1-Ig, Clone:4A2A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66921-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA PTGER4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTGER4 fusion protein Ag19262
• Vollständiger Name: prostaglandin E receptor 4 (subtype EP4)
• Berechnetes Molekulargewicht: 488 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC101534
• Gene symbol: PTGER4
• Gene ID (NCBI): 5734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.