PTGES2 Rekombinanter Antikörper

PTGES2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242930B4

Kat-Nr. : 85419-4-PBS

Synonyme

242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2

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  WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85419-4-RR (same clone as 85419-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85419-4-RR (same clone as 85419-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85419-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85419-4-RR against Human PTGES2 were performed. The affinity constant is 0.973 nM.

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Geprüfte Anwendungen

Produktinformation

85419-4-PBS bindet in WB, IF/ICC, Indirect ELISA PTGES2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PTGES2 fusion protein Ag1329
• Vollständiger Name: prostaglandin E synthase 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC011613
• Gene symbol: PTGES2
• Gene ID (NCBI): 80142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).