PTGES3 Polyklonaler Antikörper

PTGES3 Polyklonal Antikörper für FC, IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15216-1-AP

Synonyme

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 15216-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15216-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15216-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15216-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15216-1-AP

  IP Result of anti-PTGES3 (IP:15216-1-AP, 3ug; Detection:15216-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IF Staining of A549 using 15216-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 15216-1-AP (PTGES3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15216-1-AP

  Immunofluorescent analysis of HeLa cells using 15216-1-AP (PTGES3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15216-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PTGES3 antibody (15216-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Mausherzgewebe, Maushirngewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IF: A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

15216-1-AP bindet in WB, IP, IF, FC, ELISA PTGES3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTGES3 fusion protein Ag7384
• Vollständiger Name: prostaglandin E synthase 3 (cytosolic)
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 21-23 kDa
• GenBank-Zugangsnummer: BC003005
• Gene symbol: PTGES3
• Gene ID (NCBI): 10728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

Publikationen

Species	Application	Title
mouse	WB	Cell Death Differ; Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis.; Authors - Yujia Fan
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
mouse	WB	Heliyon; The inhibitory effects of Orengedokuto on inducible PGE2 production in BV-2 microglial cells.; Authors - Yoshika Iwata
human	WB	Sci Adv; PES1 is a critical component of telomerase assembly and regulates cellular senescence.; Authors - Long Cheng