COX-1/Cyclooxygenase-1 Polyklonaler Antikörper

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• KD/KO Validated

COX-1/Cyclooxygenase-1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 13393-1-AP

Synonyme

COX1, COX-1, COX-1/Cyclooxygenase-1, COX3, Cyclooxygenase 1, PCOX1, PGG/HS, PGH synthase 1, PGHS 1, PGHS1, PHS 1, PHS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, PTGHS, PTGS1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 13393-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 13393-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13393-1-AP

  IP result of anti-COX-1/Cyclooxygenase-1(IP:13393-1-AP, 4ug; Detection:13393-1-AP 1:500) with HeLa cells lysate 1280 ug.

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Neuro-2a-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13393-1-AP bindet in WB, IP, IHC, IF, ELISA COX-1/Cyclooxygenase-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX-1/Cyclooxygenase-1 fusion protein Ag4055
• Vollständiger Name: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Berechnetes Molekulargewicht: 599 aa, 69 kDa
• Beobachtetes Molekulargewicht: 60-72 kDa
• GenBank-Zugangsnummer: BC029840
• Gene symbol: PTGS1
• Gene ID (NCBI): 5742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

Publikationen

Species	Application	Title
rat	WB	Acta Pharm Sin B; Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.; Authors - Dominik Bakalarz
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse	WB	Theranostics; Nanozyme-mediated catalytic nanotherapy for inflammatory bowel disease.; Authors - Jiulong Zhao
human	WB	Free Radic Biol Med; PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis; Authors - Qiong Sun
human	WB	Acta Biomater; Localized delivery of miRNAs targets cyclooxygenases and reduces flexor tendon adhesions.; Authors - You Lang Zhou
rat	WB	Int J Mol Sci; Arachidonic Acid Metabolites of CYP450 Enzymes and HIF-1α Modulate Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats under Acute and Intermittent Hyperbaric Oxygenation.; Authors - Zrinka Mihaljević

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Thomas (Verified Customer) (09-18-2020)

HeLa cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 488 Alexa Fluor secondary antibody (1:250 - green) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HeLa

COX-1/Cyclooxygenase-1 Antibody Immunofluorescence validation (1:200 dilution) in HeLa (Cat no:13393-1-AP)