COX-1/Cyclooxygenase-1 Monoklonaler Antikörper

COX-1/Cyclooxygenase-1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F12G7

Kat-Nr. : 67346-1-PBS

Synonyme

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

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  WB analysis of L02 using 67346-1-Ig (same clone as 67346-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67346-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood leukocyte using 67346-1-Ig (same clone as 67346-1-PBS)

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67346-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67346-1-Ig (same clone as 67346-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67346-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67346-1-Ig (same clone as 67346-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67346-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67346-1-Ig (same clone as 67346-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67346-1-Ig (PTGS1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67346-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67346-1-PBS bindet in WB, IF/ICC, Indirect ELISA COX-1/Cyclooxygenase-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: COX-1/Cyclooxygenase-1 fusion protein Ag28280
• Vollständiger Name: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Berechnetes Molekulargewicht: 599 aa, 69 kDa
• Beobachtetes Molekulargewicht: 60-72 kDa
• GenBank-Zugangsnummer: BC029840
• Gene symbol: PTGS1
• Gene ID (NCBI): 5742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.