PYK2 Polyklonaler Antikörper

PYK2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10044-2-AP

Synonyme

CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

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  WB analysis of mouse brain using 10044-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10044-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10044-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10044-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human medulloblastoma using 10044-2-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 10044-2-AP (PYK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10044-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10044-2-AP (PYK2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10044-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10044-2-AP (PYK2 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Medulloblastom-Gewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10044-2-AP bindet in WB, IHC, ELISA PYK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYK2 fusion protein Ag0002
• Vollständiger Name: PTK2B protein tyrosine kinase 2 beta
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC036651
• Gene symbol: PTK2B
• Gene ID (NCBI): 2185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several introcellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis.

Publikationen

Species	Application	Title
rat	WB	Herz; Ginsenoside-Rb3 inhibits endothelial-mesenchymal transition of cardiac microvascular endothelial cells.; Authors - L Yang

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