PYK2 Monoklonaler Antikörper

PYK2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10G5

Kat-Nr. : 67141-1-PBS

Synonyme

PTK2B, 2A10G5, CADTK, CAK beta, CAKB

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  WB analysis using 67141-1-Ig (same clone as 67141-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PYK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PYK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PYK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PYK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67141-1-PBS bindet in WB, IHC, Indirect ELISA PYK2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PYK2 fusion protein Ag11487
• Vollständiger Name: PTK2B protein tyrosine kinase 2 beta
• Berechnetes Molekulargewicht: 1009 aa, 116 kDa
• Beobachtetes Molekulargewicht: 112-115 kDa
• GenBank-Zugangsnummer: BC042599
• Gene symbol: PTK2B
• Gene ID (NCBI): 2185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.