PTK6/BRK Rekombinanter Antikörper

PTK6/BRK Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242647F4

Kat-Nr. : 85352-2-RR

Synonyme

PTK6, BRK, 242647F4, Protein-tyrosine kinase 6, Protein tyrosine kinase 6

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  WB analysis using 85352-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85352-2-RR (PTK6/BRK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 85352-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using PTK6 antibody (85352-2-RR, Clone: 242647F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 85352-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PTK6/BRK antibody (85352-2-RR, Clone: 242647F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85352-2-RR bindet in WB, IF/ICC, ELISA PTK6/BRK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PTK6 protein tyrosine kinase 6
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC035843
• Gene symbol: PTK6
• Gene ID (NCBI): 5753
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRK, also named as PTK6 and FLJ42088, is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. PTK6 can phosphorylate KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. Overexpression of PTK6 in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of PTK6 has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. This antibody is specific to PTK6.