PTK7 Rekombinanter Antikörper

PTK7 Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242720A5

Kat-Nr. : 85075-1-PBS

Synonyme

242720A5, CCK 4, CCK4, CCK-4, Colon carcinoma kinase 4

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  WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85075-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85075-1-RR (same clone as 85075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85075-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01800-3

  Sandwich ELISA standard curve of MP01800-3, Human PTK7/CCK4 Recombinant Matched Antibody Pair - PBS only. 85075-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12026. 85075-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01800-3

  The mean PTK7/CCK4 concentration was determined to be 66.39 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 66.69 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load.

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  IHC staining of mouse brain using 85075-1-RR (same clone as 85075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85075-1-RR (PTK7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85075-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85075-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85075-1-RR against Human PTK7 were performed. The affinity constant is 2.60 nM.

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Geprüfte Anwendungen

Produktinformation

85075-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test PTK7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PTK7 fusion protein Ag12026
• Vollständiger Name: PTK7 protein tyrosine kinase 7
• Berechnetes Molekulargewicht: 1070 aa, 118 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC071557
• Gene symbol: PTK7
• Gene ID (NCBI): 5754
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is an atypical receptor tyrosine kinase that consists of an extracellular domain with seven immunoglobulin-like domains, a transmembrane domain, and a catalytically defective tyrosine kinase domain (PMID: 29867084). It is implicated in planar cell polarity and the Wnt canonical and non-canonical pathways (PMID: 24618420). Overexpression of PTK7 has been reported in a variety of tumors, such as cervical cancer, colon cancer, lung adenocarcinoma, acute myelogenous leukemia, and gastric cancer (PMID: 30944666).