PTPN1/PTP1B Polyklonaler Antikörper

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• KD/KO Validated

PTPN1/PTP1B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11334-1-PBS

Synonyme

PTP1B, PTPN1, EC:3.1.3.48, Protein-tyrosine phosphatase 1B, PTP 1B

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  WB analysis of Sp2/0 using 11334-1-AP (same clone as 11334-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11334-1-AP (same clone as 11334-1-PBS)

  WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  WB analysis using 11334-1-AP (same clone as 11334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11334-1-AP (same clone as 11334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11334-1-AP (same clone as 11334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 11334-1-AP (same clone as 11334-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 11334-1-AP (same clone as 11334-1-PBS)

  IP result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 11334-1-AP (same clone as 11334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11334-1-AP (PTPN1/PTP1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 11334-1-AP (same clone as 11334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11334-1-AP (PTPN1/PTP1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 11334-1-AP (same clone as 11334-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PTP1B antibody (11334-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11334-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PTPN1/PTP1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPN1/PTP1B fusion protein Ag1878
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 1
• Berechnetes Molekulargewicht: 435 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC015660
• Gene symbol: PTP1B
• Gene ID (NCBI): 5770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).