PTP4A1 Monoklonaler Antikörper

PTP4A1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C9E10

Kat-Nr. : 67584-1-PBS

Synonyme

PRL1, PTP4A1/PRL1, 2C9E10, EC:3.1.3.48, PRL-1

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  WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67584-1-Ig (same clone as 67584-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTP4A1 antibody (67584-1-Ig, Clone: 2C9E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67584-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PTP4A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTP4A1 fusion protein Ag14436
• Vollständiger Name: protein tyrosine phosphatase type IVA, member 1
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC023975
• Gene symbol: PTP4A1
• Gene ID (NCBI): 7803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).