PRL3 Polyklonaler Antikörper

PRL3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15186-1-AP

Synonyme

PRL 3, PRL R, PRL3, PTP4A3

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Galerie der Validierungsdaten

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  WB analysis using 15186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15186-1-AP (PRL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15186-1-AP bindet in WB, IHC, IF, ELISA PRL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRL3 fusion protein Ag7249
• Vollständiger Name: protein tyrosine phosphatase type IVA, member 3
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC003105
• Gene symbol: PTP4A3
• Gene ID (NCBI): 11156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphatase of regenerating liver-3 (PRL-3; also known as PTP4A3), encodes a 22-kDa protein that represents a novel class of a superfamily of protein tyrosine phosphatases (PTPs) that are ubiquitously expressed in various tumors. PRL3 was associated with primary tumor growth, tumor reoccurrence, and therapy resistance in many human cancers. This protein was also suggested as a marker to predict the relapse of gastric cancer and invasive breast cancer. (PMID: 22469786, PMID: 30157875)

Publikationen

Species	Application	Title
human	IHC	Reprod Sci; PRL-3 Is Involved in Estrogen- and IL-6-Induced Migration of Endometrial Stromal Cells From Ectopic Endometrium.; Authors - Shifan Ren
human	IF	Mol Cancer; Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma; Authors - Jianbiao Zhou