PTPIP51 Monoklonaler Antikörper

PTPIP51 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G11E2

Kat-Nr. : 68582-1-Ig

Synonyme

FAM82A2, 1G11E2, FAM82C, hRMD 3, hRMD-3

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  WB analysis using 68582-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68582-1-Ig (PTPIP51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 68582-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPIP51 antibody (68582-1-Ig, Clone: 1G11E2 ) at dilution of 1:476 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, HuH-7-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:238-1:952

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68582-1-Ig bindet in WB, IF/ICC, ELISA PTPIP51 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTPIP51 fusion protein Ag15135
• Vollständiger Name: family with sequence similarity 82, member A2
• Berechnetes Molekulargewicht: 470 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52-60 kDa
• GenBank-Zugangsnummer: BC008970
• Gene symbol: PTPIP51
• Gene ID (NCBI): 55177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618).The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).