PTPN1/PTP1B Rekombinanter Antikörper

PTPN1/PTP1B Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250215G6

Kat-Nr. : 85905-4-RR

Synonyme

PTP1B, PTPN1, EC:3.1.3.48, Protein-tyrosine phosphatase 1B, PTP 1B

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  WB analysis of MCF-7 using 85905-4-RR

  WB result of PTPN1/PTP1B antibody (85905-4-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN1/PTP1B transfected MCF-7 cells.

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  WB analysis using 85905-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85905-4-RR (PTPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85905-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN1 antibody (85905-4-RR, Clone: 250215G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85905-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85905-4-RR against Human PTPN1/PTP1B were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Jurkat-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85905-4-RR bindet in WB, IF/ICC, ELISA PTPN1/PTP1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PTPN1/PTP1B fusion protein Ag1878
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 1
• Berechnetes Molekulargewicht: 435 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC015660
• Gene symbol: PTP1B
• Gene ID (NCBI): 5770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).