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PTPN12 Polyklonaler Antikörper

PTPN12 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31215-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using PTPN12 Polyclonal antibody (31215-1-AP)

    WB analysis using 31215-1-AP (same clone as 31215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31215-1-AP (PTPN12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PTPN12 Polyclonal antibody (31215-1-AP)

    IF Staining of A431 using 31215-1-AP (same clone as 31215-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PTPN12 Polyclonal antibody (31215-1-AP)

    IF Staining of U-251 using 31215-1-AP (same clone as 31215-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31215-1-PBS bindet in WB, IF/ICC, Indirect ELISA PTPN12 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PTPN12 fusion protein Ag35382
Vollständiger Name protein tyrosine phosphatase, non-receptor type 12
Berechnetes Molekulargewicht88kDa
Beobachtetes Molekulargewicht110-125 kDa
GenBank-ZugangsnummerNM_002835.3
Gene symbol PTPN12
Gene ID (NCBI) 5782
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.