PTPN14 Polyklonaler Antikörper

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• KD/KO Validated

PTPN14 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18053-1-AP

Synonyme

PEZ, PTP36, PTPN14

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  WB analysis using 18053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18053-1-AP

  WB result of PTPN14 antibody (18053-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN14 transfected HeLa cells.

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  WB analysis of HeLa using 18053-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18053-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18053-1-AP bindet in WB, IHC, ELISA PTPN14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPN14 fusion protein Ag12571
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 14
• Berechnetes Molekulargewicht: 1187 aa, 135 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC101754
• Gene symbol: PTPN14
• Gene ID (NCBI): 5784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

Publikationen

Species	Application	Title
canine	WB	Biologicals; CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells; Authors - Zhenbin Liu